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31-07-2014, 17:06 #1
- Üyelik tarihi
- 24 Apr 14
salmonella standartı gıda
: Salmonella Standartı Gıda : .......
Isolation of Salmonella spp.
From Food and Animal Faeces
1)This procedure is based on the ISO protocol: 6579:2002 Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of Salmonella spp.4. This protocol is intended to provide guidance for the testing of suspect food items/ animal faecal specimens identified via foodborne disease surveillance programmes. Regulatory agencies (Ministries of Health, Agriculture, Commerce, etc) have specific testing requirements, different from this protocol, which much be used to test samples collected for regulatory testing (example: import/export or product recall). Prior to performing any official, legal, or regulatory testing, the reader should confirm the appropriate protocol through consult with in-country regulatory authorities.
2)This protocol is intended only to be used on food samples and animal faeces. This protocol should not be used for the testing of human faeces.
Infections due to Salmonella spp. remain a global problem. These infections may cause significant morbidity and mortality both in humans and production animals as well as considerable economic losses. Salmonella spp. are typically transmitted among humans and animals via a fecal-oral route, usually through the consumption of contaminated food or water. Timely identification and serotyping of Salmonella from clinical specimens facilitates outbreak detection and patient management while prompt and accurate detection of Salmonella spp. in contaminated food or water provides an opportunity to prevent the contaminated food from entering the food supply.
Sensitive and specific laboratory methods for the isolation, identification, and serotyping of Salmonella are essential elements of Salmonella monitoring and control programmes. An ideal method will be rapid, inexpensive, easily reproducible, sensitive, and specific. Currently, no single method meets all these criteria and the optimal method may vary depending on the source of specimen (e.g., human clinical specimens, different food matrices, and environmental specimens) and the target serotype (e.g. typhoidal versus non-typhoidal Salmonellae). Additionally, new methods are being described regularly and comparison of current methodologies to new methodologies is highly recommended. To insure continuity of results, any new method must be validated and standardized prior to implementation.
The following protocol is based on the ISO-6579 standard method for the isolation and identification of Salmonella from food and animal faeces. This procedure has been extensively documented in the peer-reviewed literature and has been accepted by international accreditation agencies. It can be tailored to fit the needs of most laboratories world-wide. Molecular methodologies have the potential to increase sample throughput, sensitivity, and specificity, while simultaneously reducing turnaround time. However, it is important to utilize the appropriate test for the appropriate sample, for example PFGE can provide supplementary discrimination below the level of serotype, however it should not take the place of serotyping. Also, many molecular assays are non-culture tests (or only require pre-enrichment). While these assays may provide rapid screening results, it is essential that an attempt be made to obtain an isolate for additional studies such as serotyping and susceptibility testing.
Detailed identification and serotyping procedures may be found in the Global Salm-Surv Laboratory Protocol Identification of Salmonella and Shigella Using an Abbreviated Panel of Tests7 and Serotyping of Salmonella and a flow diagram providing a brief overview of the isolation, identification, and serotyping procedure may be found in Appendix 3. 2
1. Important Notes and Forward........................................... ....................................1
1.1 Procedure for Isolation of Salmonella from food and animal faeces..................4-8
2. Composition and preparation of culture media and reagents..........................9-13
Appendix 1. Flow Diagram for Isolation of Salmonella from food and animal faeces..14
Appendix 2. Photographs of Salmonella and other organisms on various media...15-16
Laboratory record sheets............................................ .........................................17-18
Konu Çiğdem Coşkun tarafından (31-07-2014 Saat 17:36 ) değiştirilmiştir.
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